Composite
Part:BBa_K3396009:Design
Designed by: Yuxin Liu Group: iGEM20_NUDT_CHINA (2020-10-25)
GFPnano-DocS-P2A-Trim21-Coh2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1790
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1747
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 721
Design Notes
We set this part to realize expressing BBa_K3396005 and BBa_K3396006 into the same cell through transforming only one kind of plasmid.
Source
The original Trim21 is expressed in human. We did the truncation based on the protein domain information given in Uniprot. Both DocS and Coh2 originally expressed in C. thermocellum scaffoldin. The sequence of GFP-nano we use came from a patent. P2A is used to express two peptides with only one RBS.